Plant viral movement proteins: Agents for cell-to-cell trafficking of viral genomes

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Plant viral movement proteins: agents for cell-to-cell trafficking of viral genomes.

Plants viruses spread throughout their hosts using a number of pathways, the most common being movement cell to cell through plasmodesmata (PD), unique intercellular organelles of the plant kingdom, and between organs by means of the vascular system. Pioneering studies on plant viruses revealed that PD allow the cell-to-cell trafficking of virally encoded proteins, termed the movement proteins ...

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Effects of calreticulin on viral cell-to-cell movement.

Cell-to-cell tobacco mosaic virus movement protein (TMV MP) mediates viral spread between the host cells through plasmodesmata. Although several host factors have been shown to interact with TMV MP, none of them coresides with TMV MP within plasmodesmata. We used affinity purification to isolate a tobacco protein that binds TMV MP and identified it as calreticulin. The interaction between TMV M...

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Identification of a Functional Plasmodesmal Localization Signal in a Plant Viral Cell-To-Cell-Movement Protein

UNLABELLED Our fundamental knowledge of the protein-sorting pathways required for plant cell-to-cell trafficking and communication via the intercellular connections termed plasmodesmata has been severely limited by the paucity of plasmodesmal targeting sequences that have been identified to date. To address this limitation, we have identified the plasmodesmal localization signal (PLS) in the To...

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GAPDH-A Recruits a Plant Virus Movement Protein to Cortical Virus Replication Complexes to Facilitate Viral Cell-to-Cell Movement

The formation of virus movement protein (MP)-containing punctate structures on the cortical endoplasmic reticulum is required for efficient intercellular movement of Red clover necrotic mosaic virus (RCNMV), a bipartite positive-strand RNA plant virus. We found that these cortical punctate structures constitute a viral replication complex (VRC) in addition to the previously reported aggregate s...

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application of suspension cell culture for preparation of viral antigens

introduction: hela cell is a widely studied cell line derived from human cerzix adenocarcinoma. the cells are epithelial - like in morphology and susceptible to polio viruses and adenovirus type 5. hela cells are usually as monolayer for isolation and propagation of some human viruses, as well as for the expression of recombinant proteins, including hepatitis b surface antigen. the aim of this ...

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ژورنال

عنوان ژورنال: Virology

سال: 2006

ISSN: 0042-6822

DOI: 10.1016/j.virol.2005.09.026